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KMID : 1094720160210030414
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Biotechnology and Bioprocess Engineering
2016 Volume.21 No. 3 p.414 ~ p.421
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Fed-batch fermentation of indole-3-acetic acid production in stirred tank fermenter by red yeast Rhodosporidium paludigenum
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Nutaratat Pumin
Srisuk Nantana
Arunrattiyakorn Panarat
Limtong Savitree
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Abstract
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Indole-3-acetic acid (IAA) is a significant secondary metabolite that is the most important auxin of plant hormones. Production of IAA is considered to be a key trait to support plant growth. The improvement of IAA production by a basidiomycetous red yeast Rhodosporidium paludigenum DMKU-RP301 was investigated. Batch and fed-batch fermentation of R. paludigenum DMKU-RP301 were conducted in a 2 L stirred tank fermenter. Using batch fermentation, it was found that when cultivated at an agitation speed of 200 rpm and a 3 L/min aeration rate, this yeast produced IAA at its maximum level of 1,627.1 mg/L (9.7 mg/L/h). In fed-batch fermentation, a higher level of maximum IAA production than that found in batch fermentation was observed, i.e. 2,743.9 mg/L (25.4 mg/L/h). It is therefore suggested that fed-batch fermentation improves the efficiency of IAA production in terms of product concentration and IAA productivity. Moreover, yeast carotenoid production was also investigated using R. paludigenum DMKU-RP301, and found a maximum carotenoid production of 3.05 mg/L.
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KEYWORD
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batch fermentation, fed-batch fermentation, indole-3-acetic acid, Rhodosporidium, yeast
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